Single-Label Plate Reader: SpectraMax Plus 384 (Molecular Devices). The optical systems use two scanning monochromators soUnlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. I've got a LT4500 microplate reader and was hoping to digest the pellets in papain overnight, simply mix a measure of that sample with DMMB solution in a 96 well plate and read absorbance at 525nm. The chamber. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. SpectraMax Glo Steady-Luc Reporter Assay Kit workflow. It is upgradeable to include a barcode reader, programmable injectors, and dual-channel configurations for a 2-fold increase for medium throughput. Microplate readers’ standard detection modes. E Models. Tristar 3 Multimode Microplate Reader. Figure 2 shows a Lck kinase dilution curve obtained using the FAM-p34cdc2-derived peptide. Appendix A. The SpectraMax iD3 reader detects fluorescence using monochromators only. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. Plate Shaking Linear, orbital, double-orbital Dimensions (H x W x D) 12 x 15 x 15 in. 00. 1. On the SpectraMax i3x reader, HTRF (cartridge) should be selected as the Optical Configuration. Test Plates for absorbance and fluorescence intensity provide a complete traceable solution for validating optical performance of the SpectraMax M2 reader, automatically. The SpectraTest absorbance test plate (Abs-1) can be used by VersaMax, SpectraMax, & M2 readers. The SpectraMax M2 and SpectraMax M2e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as. The cooled PMT detector allows high sensitivity in a wide dynamic range, even in extremely low light. The SpectraMax® ABS Plus Microplate Reader can run . Top reading usually provides better signal-to-noise ratios for solution-based assays such as DNA quantification or protein quantification. Package performance tests. Whether fluorescence, chemiluminescence, or visible detections - select the optimal plate for your assay!If your microplate reader requires an adapter for top reading (e. Data fromSpectraMax® M2 Multi-Mode Microplate Reader MLDVM2 SpectraMax® M2e Multi-Mode Microplate Reader (top and bottom reading) MLDVM2E. 4. USING OLIGREEN REAGENT IN GEMINI XS, GEMINI EM AND SPECTRAMAX M2 MICROPLATE READERS Step 6: Add 100 µL of each concentration of oligonucleotide to the wells of the microplate in triplicate. free tracer decreases, resulting in an overall increase in RFU values. 00. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers. • SpectraMax® M2/M2e/M3/M4/M5/M5e Multi-Mode Microplate Readers • SpectraMax® Paradigm® Multi-Mode Microplate Reader • FlexStation® 3 Multi-Mode Microplate Reader • Gemini™ EM/XPS Fluorescence Microplate Readers • FilterMax™ F3/F5 Multi-Mode Microplate Readers Table 1. Based on the SpectraMax M5e platform, this five-mode reader addresses multiple application technologies including: absorbance, fluorescenceSeller assumes all responsibility for this listing. g. Fluorescence Microplate Reader Comparison Chart. For example, microplates containing different samples can be read using the Tecan Infinite® M1000 Pro Plate Reader. Offer Ends In: 45 Days 06: 20: 11. Fully tested with validations and warranty. 04 to 3. The Biophysical Resource at Keck Facility offers the fluorescence plate reader as an "open access" instrument that can be used by trained users. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. 2018. 7. Microplate Reader Shelf. 9000-0756. These instruments often feature additional detection modes in addition to fluorescence intensity, such as UV-vis absorbance, fluorescence. Featuring temperature control up toThe SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. 020 OD 2. View online or download Molecular devices SpectraMax M3 User Manual. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. Shop now. The microplates were read the next day from the bottom and the top in SpectraMax M5 and Gemini EM readers. BioTek Synergy HTX Microplate Reader - Multi Mode (Abs + FL + Lum), Bio-tek, Used plate reader, BioTek multi-mode detection, PMT for luminescence detection, kinetic assays, Time Resolved Fluorescence, Gen5 software, absorbance reader, fluorescence detection, used lab. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. 5039228D 3 Contents SafetyInformation 5 Warnings,Cautions,Notes,andTips 5 Chapter1:Introduction 11 ComputerIntegration 12 Applications 12 Optics 13 PlateControls 13Fluorescence Microplate Readers. Molecular Devices Spectra Max Gemini XS fluorescence Microplate reader. Read More. Assay plates containing the 15-point standard curve were read on the Molecular Devices' SpectraMaxš M2 Microplate Reader. 209, but in 96-well plate was 0. The data acquisition and analysis is easily streamlined when using the preconfigured protocol in Softmax Pro Sofware. Add to Cart. WinKQCL ® Software guides the user through the process of setting up a microplate template and running an assay to determine the amount of endotoxin in a sample solution. Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. On microplate readers,. ID: MOLECULAR DEVICES SpectraMax M2 is a multi-mode microplate spectrophotometer providing unbeatable accuracy, dependability and versatility for any lab experiment, with a range of detection modes, advanced accessories, and intuitive software. The BioTek Synergy H1 multimode microplate reader is modular and upgradable to include variable-bandwidth monochromator optics for fluorescence excitation and emission wavelength selection, plus luminescence and UV-Vis absorbance. By employing dyes that specifically bind to nucleic acids, DNA/RNA concentrations can also be quantified using fluorescence detection. Microplate Formats: 6, 12, 24, 48, 96, 384. 10. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western. Avoid losing high signal. 0 × 103 to 2. The Cut-off filter for this pair is showing 530nm in this machine. Selecting your dsDNA. The Molecular Devices Gemini EM is built on the Gemini platform delivering high quality top and bottom reading for both solution and cell based assays. 67. Microplate Readers. Fluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. The SpectraMax i3x reader provides theThis Molecular Devices SpectraMax iD5 Microplate Reader is made in Oct. 1. The plate is set up for reading and images are acquired according to specified parameters. 2 or higher, standard edition only, no GxP) VersaMax™ Microplate Reader (Serial numbers begining with BNxxxxx and BNRxxxxx are supported) SpectraMax® 190 Absorbance Microplate Reader (Serial. Microplate Reader Shelf. The reader adds up to three transfers of assay reagents from a 96- or 384-well source plate to enhance assay design using integrated fluidics. Microplate Readers Molecular Devices Spectramax iD3 Microplate Reader. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. 3) At the bottom of the strip, click on "Show. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Test Plates for absorbance and fluorescence intensity provide a complete traceable solution for validating optical performance of the SpectraMax M2 reader, automatically. 314 low lamp level warning. Double-click the unknown device with the yellow warning icon. A SpectraMax M2 microplate reader helps minimize operating time and save costs, allowing researchers to spend more time analyzing data and generating practical knowledge. , contact. SpectraMax Gemini EM Microplate Reader A dual-monochromator spectrofluorometer system Dual monochromators. 次いで、ユーザーのラボにとって最も役立つ機能を有するモデルを自由に. The Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. M2. The modular platform allows upgrading to expand functionality. SpectraMax M2. begin reading microplates with seven mouse clicks. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. was 1. After the plate is read, data will appear in the plate. 0) at 37 °C, fluorescence intensity was recorded on a Spectramax M5 Multimode plate reader. 40266 Email: drugdiscoverytech@lifetech. The plates were then read with the SpectraMax M3 plate reader (Molecular Devices, Sunnyvale, CA) to obtain absorbance readings at 570 nm. Specialized microplate readers for luminescence detection are available for use in applications such as luciferase reporter assays, bioluminescence resonance energy transfer (BRET), and other luminescent assays. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. Gemini XPS Fluorescence Microplate Reader . This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. duAl monochromAtors With SpectraMax M2/M2e. However, proNGF-induced receptor complex formation has been difficult to directly assess other than by western blotting. type: Clear: Add to Cart. Plate chamber design of the SpectraMax category 1 microplate readers. Type Clear: Add to Cart. Auto-calibration and self-diagnostics. Figure 1. fluorescence intensity, time-resolved fluorescence (M4, M5, M5 e models only) or wavelength-selectable luminescence modes, and 400–750 nm for readings in fluorescence polarization mode (M5, M5e models only). SpectraMax Plus, Plus384, M2, M2e, M5e or M5 readers). . Screen Snapshot of Settings Dialog Box As the ratio of ADP:ATP increases, the proportion of bound tracer vs. (a) Chemical structures of 1 (HADP) and its analogues 2–4. They are widely used in research, drug discovery, [1] bioassay validation, quality control and manufacturing processes in the pharmaceutical. (a) Chemical structures of 1 (HADP) and its analogues 2–4. iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, FlexStation 3, Gemini EM, and Gemini XPS readers. e. The ultimate guide to microplate readers is an eBook that tells you everything you need to know about plate reader solutions from what a microplate reader is, to popular applications and technology, to GxP compliance and special tips and tricks to help maximize your. Other technologies such as imaging. The OliGreen assay from Molecular Probes, when run on a Gemini XS or Gemini EM fluorescence microplate reader with SoftMax Pro software, is a quick, sensitive detection method for single-stranded DNA. KEY FEATURES • Use the touchscreen to easily set up methods, runSpectraMax® i3xMulti-ModeMicroplateReader SpectraMax® i3Multi-ModeMicroplateReader FlexStation® 3Multi-ModeMicroplateReader SpectraMax® M5eMulti-ModeMicroplateReader SpectraMax®M5Multi-ModeMicroplateReader SpectraMax® M4Multi-ModeMicroplateReader SpectraMax® M3Multi. Because. 2 % of reading SpectraMax Plus384 microplate reader and absorbance plate ABS1 SpectraMax ABS Plus microplate reader and Absorbance plate. Multimode Microplate Readers. Our absorbance plate readers feature our PathCheck Sensor technology and. olecular saka. 1. Optimal excitation APPLICATION NOTE and emission wavelengths for SpectraMax M5 fluorescence. 25 fmol/well 384-well plate) Filters/Mirrors: Fluorescein 1 pM typical (0. Molecular Devices SpectraMax M2. Page 28 SpectraMax iD3 Multi-Mode Microplate Reader User Guide 4. Gemini XPS & EM readers measure fluorescence on a variety of sample formats from 6- to 384-well. Test Plates for absorbance and fluorescence intensity provide a complete traceable solution for validating optical performance of the SpectraMax M2 reader, automatically. 3: The cuvette chamber. MPN: 50505. D 59 A. SpectraMax Quant dsDNA Assay Kits. Set up a plate section for a second emission scan as in Step C above, except for the added Emission Cutoff Filter. Compatible automation solutions for SpectraMax Readers. Results were graphed in SoftMax Pro Software using a 4-parameter curve fit. fluorescence). Standard 6-, 12-, 24-, 48-, 96- and 384-well microplates. The fluorescence was measured in the SpectraMax M3 Microplate reader. 67. SpectraMax i3x Multi-Mode Microplate Reader measures absorbance, fluorescence and luminescence. (b) Fluorescence responses of probe HADP and control compounds 2–4 (each 5 µM) towards human heparanase (HPA) (1 µg) in 40 mM NaOAc buffer (pH 5. Item Description Sensitivity 10amol/wellalkalinephosphate200µL Wavelengthrange 250-850nm Crosstalk <0. (30. A SpectraMax M2 microplate reader helps minimize operating time and save costs, allowing researchers to spend more time analyzing data and generating practical knowledge. 1. SpectraMax M2 and SpectraMax M2e from Molecular Devices are multi- detection microplate readers with dual-monochromators, dual-mode cuvette ports and top- and bottom-reading capability (top-reading only on SpectraMax M2). Learn how you can utilize microplate readers to drive landmark discoveries. : 2 Oct 12 Page 1 of 20 Setup Guide on the Molecular Devices SpectraMax® Paradigm® Microplate Detection Platform Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. Sale. The high sensitivity, low-noise photomultiplier tubes (PMTs), and variable-volume. Page 1 SpectraMax M2/M2 user guide ® A MULTI-DETECTION MICROPLATE READER WITH TWO-MODE CUVETTE PORT. Skip to main content. 0) OD (0. Enables accurate fluorescence data with every run. of each sample medium was transferred to a 96-well flat-bottom plate. I understand the OD measurement in a plate reader varies with the volume of the sample measured. In addition, combined absorbance and fluorescence intensity assays can be run by issuing a single read command. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard plate reader applications, protecting your initial investment. SpectraMax Paradigm Multi-Mode Safety Guide The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. 100 readings per well gives optimal Z’ values for this assay. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. For fluorescent assays, black plates absorb light and reduce background and crosstalk. (b) Fluorescence responses of probe HADP and control compounds 2–4 (each 5 µM) towards human heparanase (HPA) (1 µg) in 40 mM NaOAc buffer (pH 5. An assay plate containing a 15-point standard curve was read on the SpectraMax. A microplate reader asPython 3. The systems have. Description 1. 304 signal level saturation. dual monochRomatoRs foR assay flexibility With SpectraMax Multi-Mode Readers, there is no need to utilize expensive filters to optimize detection levels and background. Features. (in addition to fluorescence imaging) on the SpectraMax i3x Multi-Mode Microplate Reader. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. 0 out of 5 $ 3,895. Other technologies such as. 2 % of reading SpectraMax Plus384 microplate reader and absorbance plate ABS1 SpectraMax ABS Plus microplate reader and Absorbance plate. The dual monochromator optics allow the. 25 to 3. Intuitive software and help tools45 s, and a 1,536-well plate in 135 s (minimum times) Interface. Documentation:Plate readers that are used to detect luminescence, fluorescence and absorbance signals can vary in sensitivity (Figure 2). The SpectraMax Quant dsDNA Assay Kits provide pre-optimized, complete solutions that are sensitive, accurate, and easy to use. It features a modular design that allows users to add or change detection cartridges according to their needs. Luminescence was read on the SpectraMax i3x reader and the SpectraMax L reader. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. It is important to determine the excitation and emission wavelengths that provide the best dynamic range for the assay, since these may differ somewhat from those used with filter-based plate readers. Fig 2: Enzymatic response of synthetic compounds. Molecular Devices SpectraMax™ M2e Microplate Reader w/ Warranty. Figure 2-23: Bottle holder To install the bottle holder: 1. : 8 Oct 12 Page 7 of 10 Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. 3) At the bottom of the strip, click on "Show. Description 1. SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. 1 SoftMax Pro v4. Model Description. Fill a clean plate with water. Analyze samples using fluorometry, photometry, luminometry, TRF or FP with these. Plate stacker and robot integration The SpectraMax M2 and M2e readers can be integrated with the A microplate reader that can detect two or more applications is considered a multi-mode plate reader. Item Description Sensitivity 10amol/wellalkalinephosphate200µL Wavelengthrange 250-850nm Crosstalk <0. The analysis capabilities of SoftMax Pro Software provide quantitation in an easy-to-read, user-customizable report format. g. Linear, Orbital Slow, and Orbital Fast. SpectraMax M2, M2e;. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. Biotek Synergy 2 Plate Reader Multi-Mode; Related products. Molecular Devices SpectraMax M2 Microplate Reader. 53 - 60 kg, (117-132 lb), depending on configuration. 6 % of reading 4. 0 × 103 to 2. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. Molecular Devices SpectraMax M2 ; Molecular Devices. DNA quantification with fluorescence dyes. Step 4: Use the Template Editor to assign wells to ‘Standards’ and ‘Unknowns’ groups (Figure 2). Add to Cart. SpectraMax M5), place the adapter in the plate carriage. Self Service Available. SoftMax Pro. Real-time experimental control (REC) that automatically activates a procedure as soon as a specific data value is reached. New Life Scientific backs all orders with a 90-day warranty. 45 s, and a 1,536-well plate in 135 s (minimum times) Interface. Typical settings forTo maximize sensitivity of the assay, it is necessary to use optimal excitation and emission wavelengths. In the M2, M5 and Plus. 6% for the on-board optics (monochromators). 1) A maximal fluorescence intensity of 177 ± 3 RFU was observed at the highest cell concentration (Table 1). . 1. TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode. It allows you to explore cellular pathways and protein activation and expression in one system. B. Table 1. The Spectramax i3 is a muti-mode plate reader used for measuring absorbance and fluorescence in microtiter plates. Temperature variation ± 0. Flexibility to read 6- to 384-well plates. Plate background OD values of disposable UV microplates vary slightly between lots. It is also extremely flexible and can measure samples held in the 96, 384, 1536 and other. For fluorescence intensity, time resolved fluorescence, and fluorescence polarization assays, the SpectraMax M5 Microplate Reader optical design provides the highest level of flexibility. The following general steps are taken with our absorbance and fluorescence readers. 0 out of 5 $ 2,999-20%. Perform the scan and note the wavelength that gives maximum emission. Used. Model: SpectraMax M2. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. Thermo Fluoroskan Ascent FL Fluorescence Microplate Reader. Plate reader set to 50 reads. For a complete list of the instruments supported by this release of theThe HTRF Reader Control Kit provides an easy and straightforward way to assess your reader’s overall performance and can facilitate the identification of potential limitations. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Using a black. Tristar 3 Multimode Microplate Reader. 2. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. Page 28 Chapter 5: Read Modes and Read Types 1. SpectraMax readers are equipped with PathCheck Technology, which measures the pathlength in each well of a microplate and normalizes the absorbance. Equipment Details. In addition, the SpectraMax iD5The SpectraMax ® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. 5. Product Information. The instrument is located in room 2131 at 300 George Street. The UV-Vis SpectraMax 190 Microplate Reader for Quantification of DNA and endpoint, kinetic, and spectral scanning analyses. 4. Microplate Reader, Multimode. Allows you to choose your sample format depending on your unique research and throughput needs. Multimode Readers Comparison Chart. Multimode microplate readers deliver both performance and economy. 9000-0756. made for this assay plate. The plate reader optics are tunable for absorbance. $22,500. 87. Also refer to this Multimode Reader Comparison Chart. Whether you need to measure fluorescence, absorbance, luminescence, or time-resolved fluorescence (TRF), or you use PerkinElmer ™ AlphaScreen. The SpectraMax® L Microplate Reader measures flash and glow luminescence assays in both 96- and 384-well plates. Manufacturer: Molecular Devices; Model: SpectraMax; The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2. Type Clear: Add to Cart. Customer Satisfaction is our number one goal All lab equipment in stock already tested and ships in 1-3 days The plates were then read with the SpectraMax M3 plate reader (Molecular Devices, Sunnyvale, CA) to obtain absorbance readings at 570 nm. Similar settings will work for other SpectraMax readers with fluorescence detection. The SpectraDrop Micro-Volume microplate offers the ability to use as little as 2 µL samples with 24 or 64-well plates. The SpectraMax iD5 reader rapidly performs absorbance measurements that are used to plot standard curves and calculate sample concentrations using a preconfigured protocol in SoftMax Pro Software. 0 × 103 to 2. UsethecuvetteportforAbsorbance,Fluorescence,andLuminescence(secondary mode)readmodes. Spectramax Multi-Mode Microplate Reader Performance Specifications. D. 6 % of reading 4. In addition, the SpectraMax iD5SpectraMax Microplate Readers. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. Page 2 SpectraMax ® SpectraMax ® Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part #0112-0102 Rev. We would like to show you a description here but the site won’t allow us. item 1 Molecular Devices SpectraMax M2 Multi-Mode Microplate Reader - JVSHJH O Molecular Devices SpectraMax M2 Multi-Mode Microplate Reader - JVSHJH O $4,999. While many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high degree of feature variability. For problems with SpectraMax Multi-Mode Microplate Readers that are not listed here, in the U. Transcreener ® ADP. Furthermore, VersaMax system is reliable, which means more uptime and lower long-term cost of ownership. Test Plates for absorbance and fluorescence intensity provide a complete traceable solution for validating optical performance of the SpectraMax M2. Instruments are configurable for the assay-specific detection modes and performance your laboratory needs. For more sample throughput, the system canProducts : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, SpectraMax Plus 384 reader, SpectraMax 190. Assays requiring a read in two or more modes can be combined and run on the SpectraMax Multi-Mode Microplate Readers by issuing. Fluorescence measurements were acquired with a Molecular Devices SpectraMax M2 Microplate Reader using 96 well plates, with the samples being excited at 468 nm and emission being measured at 572 nm. 7°C (gradient heating design). Download PDF. SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. The entry should be SpectraMax GEMINI EM. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. A microplate reader as individual cuvettes (using SpectraMax Plus, Plus384, M2, M2e, M5e or M5). Three data types for FP read types are also being introduced with v4. The SpectraDrop Micro-Volume microplate offers the ability to use as little as 2 µL samples with 24 or 64-well plates. ) Identical data and curves were generated from Gemini EM and SpectraMax M2 but are not shown here. Step 5: Click the software’s Read button. Measure more accurately. It combines the sensitivities of filters with the convenience of a monochromator to provide the complete laboratory solution with absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. Multi-Mode Microplate Readers including the M3, M4, M5, and M5. assays, we can offer a microplate reader solution to help meet the. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. The SpectraDrop Micro-Volume microplate offers the ability to use as little as 2 µL samples with 24 or 64-well plates. Parameter EarlyTox Live/Dead EarlyTox Glutathione EarlyTox Caspase-3/7 R110 Read mode Fluorescence Fluorescence Fluorescence Read type Endpoint Endpoint Endpoint Wavelengths Lm1:. When using the PyroWave XM Reader, excitation wavelengths of 380 nm and emission wavelengths of 440 nm are used to measure fluorescence. The LVis Plate is transparent in the UV-range of light and is compatible with all BMG LABTECH microplate readers. Instrument design, user interface capabilities, and cost play key roles in purchasing a suitable microplate reader. 49 µM,. Molecular Devices SpectraMax M2 ; Molecular Devices. BMG Labtech - THERMOstar. The Molecular Devices Gemini EM is built on the Gemini platform delivering high quality top and bottom reading for both solution and cell based assays. 筛选时，SpectraMax M5多功能微孔板读板 机可作为其非常理想的、可靠的检测平台。 SpectraMax M5是一种基于光栅型单色器 的多功能微孔板读板机，允许使用者针对不 同的荧光染料分子随意选择不同的检测波 长，而无需额外再选购滤光片配件。这篇应 Instrument settings used with the SpectraMax M5 Microplate Reader. U. 5%in96-and384-wellmicroplates. Assays requiring a read in two or more modes can be combined and run on the SpectraMax Multi-Mode Microplate Readers by issuing. When a. Basic "FL Plate Reader start up protocol" (print and bring along for the. Readers – Photometric Accuracy Fluorescence Intensity Luminescence Intensity (0. SpectraMax M2 and M2e Performance Specifications SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. Learn how to operate the VersaMax, SpectraMax 340PC384 and SpectraMax 190 Plus384 microplate readers, which offer fast and accurate absorbance measurements for a variety of assays. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v1. . The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. FL1 Fluorescence Plates, and LM1 Luminescence Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. NanoQuant plate. SpectraMax Plus, Plus384, M2, M2e, M5e or M5 readers). 筛选时，SpectraMax M5多功能微孔板读板 机可作为其非常理想的、可靠的检测平台。 SpectraMax M5是一种基于光栅型单色器 的多功能微孔板读板机，允许使用者针对不 同的荧光染料分子随意选择不同的检测波 长，而无需额外再选购滤光片配件。这篇应Instrument settings used with the SpectraMax M5 Microplate Reader. Plate reader set to 50 reads. Analyze the data Step 1: After the microplate has been read, theFluorescence analysis was performed using a SpectraMax iD3 multi-mode plate reader (Molecular Devices LLC, U. Python 3. 60 SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. 0 software was used to handle data analysis and control each instrument. You should use solid white plates for top Time- Resolved Fluorescence reads, and white plates with clear bottoms for bottom reads. 119. SpectraMax M2 and use thereof is covered by issued (U. Third this recommendation.